LC/MS/MS Analysis of Oral Anti-diabetes Drugs (VC-50 2D)

Acarbose, miglitol and voglibose are amino sugars each possessing amino groups, and α-glucosidase inhibitors which have been used as oral anti-diabetes drugs. Metformin contains multiple amines and falls under the biguanide drug family; which is known as oral anti-diabetes drug. In this application, those drugs and metformin were simultaneous analyzed using HILICpak VC-50 2D, a polymer-based column for HILIC mode.

Sample: 5 µL
5 ng/mL each (in H2O)

  1. Acarbose
  2. Miglitol
  3. Voglibose
  4. Metformin
Column
：Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm)
Eluent
：(A); 200 mM HCOOH aq. (B); CH3CN
Linear gradient (High pressure); B % = 60 % (0 to 5 min), 60 % to 20 % (5 to 6 min), 20 % (6 to 20 min)
Flow rate
：0.3 mL/min
Detector
：ESI-MS/MS (MRM Positive)
Column temp.
：40 ℃

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