Acarbose, miglitol and voglibose are amino sugars each possessing amino groups, and α-glucosidase inhibitors which have been used as oral anti-diabetes drugs. Metformin contains multiple amines and falls under the biguanide drug family; which is known as oral anti-diabetes drug. In this application, those drugs and metformin were simultaneous analyzed using HILICpak VC-50 2D, a polymer-based column for HILIC mode.


Sample :
5 ng/mL each (in H₂O), 5 μL
Acarbose
Miglitol
Voglibose
Metformin

Column       : Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm)
Eluent       : (A) 200 mM HCOOH aq. 
               (B) CH3CN    
               Linear gradient (High pressure) ;
               B % = 60 % (0 to 5 min) 
                    60 % to 20 % (5 to 6 min)
                    20 % (6 to 20 min) 
Flow rate    : 0.3 mL/min
Detector     : ESI-MS/MS (MRM Positive)
Column temp. : 40 °C