Acarbose, miglitol and voglibose are amino sugars each possessing amino groups, and α-glucosidase inhibitors which have been used as oral anti-diabetes drugs. Metformin contains multiple amines and falls under the biguanide drug family; which is known as oral anti-diabetes drug. In this application, those drugs and metformin were simultaneous analyzed using HILICpak VC-50 2D, a polymer-based column for HILIC mode.
5 ng/mL each (in H2O), 5 μL
Column : Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm) Eluent : (A) 200 mM HCOOH aq. (B) CH3CN Linear gradient (High pressure) ; B % = 60 % (0 to 5 min) 60 % to 20 % (5 to 6 min) 20 % (6 to 20 min) Flow rate : 0.3 mL/min Detector : ESI-MS/MS (MRM Positive) Column temp. : 40 °C
Sample Name Index
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