Local Anesthetics (2) (DE-413)

Local anesthetics were analyzed using RSpak DE-413, a column for reversed phase chromataography, with alkaline eluent.
Please refer to Analysis of Short Amines without Using Ion Pair Reagent.

Sample : 10 μL
1. Benzocaine 20 μg/mL
2. Lidocaine 100 μg/mL
3. Tetracaine 100 μg/mL

Column       : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent       : 20 mM Sodium phosphate buffer (pH9.0)/CH3CN=50/50
Flow rate    : 0.8 mL/min
Detector     : UV (254 nm)
Column temp. : 40 °C

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Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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