Local anesthetics were analyzed using RSpak DE-413, a column for reversed phase chromataography, with alkaline eluent.
Please refer to Analysis of Short Amines without Using Ion Pair Reagent.


Sample : 10 μL
1. Benzocaine 20 μg/mL
2. Lidocaine 100 μg/mL
3. Tetracaine 100 μg/mL

Column       : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent       : 20 mM Sodium phosphate buffer (pH9.0)/CH3CN=50/50
Flow rate    : 0.8 mL/min
Detector     : UV (254 nm)
Column temp. : 40 °C