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Local Anesthetics (2) (DE-413)


Local anesthetics were analyzed using RSpak DE-413, a column for reversed phase chromataography, with alkaline eluent.
Please refer to Analysis of Short Amines without Using Ion Pair Reagent.

Sample : 10μL
1. Benzocaine 20μg/mL
2. Lidocaine 100μg/mL
3. Tetracaine 100μg/mL

Column       : Shodex RSpak DE-413 (4.6mmI.D. x 150mm)
Eluent       : 20mM Sodium phosphate buffer(pH9.0)/CH3CN=50/50
Flow rate    : 0.8mL/min
Detector     : UV(254nm)
Column temp. : 40°C

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