Analysis of Macrolide Antibiotics and Derivatives (ODP-50 4D)

Asahipak ODP-50 4D, a reversed phase chromatography column, was used for simultaneous analysis of erythromycin, a macrolide antibiotics, and its derivative azithromycin. Since macrolide antibiotics are basic substances, their dissociation is suppressed under alkaline conditions, and this makes them easily retained in reversed phase analysis. ODP-50 4D has an excellent  durability towards alkaline conditions, and thus enables this analysis.

Sample: 0.1 % each, 10 μL

  1. 1.Erythromycin
  2. 2.Azithromycin
Macrolide_antibiotics_chromatogram
Column
：Shodex Asahipak ODP-50 4D (4.6 mm I.D x 150 mm)
Eluent
：40 mM Potassium phosphate buffer (pH11.0)/CH3CN=40/60
Flow rate
：0.5 mL/min
Detector
：UV (223 nm)
Column temp.
：40 ℃

The reversed phase Asahipak ODP-40 4E column was used here to analyze simultaneously the macrolide antibiotic Erythromycin and its derivative Azithromycin. The polymer-based ODP-40 4E column is very alkaline-resistant and can therefore be used under alkaline conditions.
*Sales of the product used in this application is discontinued, however if you are still interested in this application, please contact us.


Sample : 10 μL
0.1 % each
1. Erythromycin
2. Azithromycin

  
Column       : Shodex Asahipak ODP-40 4E (4.6 mm I.D. x 250 mm)
Eluent       : 40 mM Potassium phosphate buffer (pH11.0)/CH3CN=40/60
Flow rate    : 0.5 mL/min
Detector     : UV (223 nm)
Column temp. : 40 °C

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