Macrolide Antibiotics and Derivatives (ODP-40 4E)

The reversed phase Asahipak ODP-40 4E column was used here to analyze simultaneously the macrolide antibiotic Erythromycin and its derivative Azithromycin. The polymer-based ODP-40 4E column is very alkaline-resistant and can therefore be used under alkaline conditions.
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Sample : 10 μL
0.1 % each
1. Erythromycin
2. Azithromycin

Column       : Shodex Asahipak ODP-40 4E (4.6 mm I.D. x 250 mm)
Eluent       : 40 mM Potassium phosphate buffer (pH11.0)/CH3CN=40/60
Flow rate    : 0.5 mL/min
Detector     : UV (223 nm)
Column temp. : 40 °C

Sample Name Index


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