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Macrolide Antibiotics and Derivatives (ODP-40 4E)


The reversed phase Asahipak ODP-40 4E column was used here to analyze simultaneously the macrolide antibiotic Erythromycin and its derivative Azithromycin. The polymer-based ODP-40 4E column is very alkaline-resistant and can therefore be used under alkaline conditions.

Sample : 0.1% each, 10μL
1. Erythromycin
2. Azithromycin

Column       : Shodex Asahipak ODP-40 4E (4.6mmI.D. x 250mm)
Eluent       : 40mM Potassium phosphate buffer(pH11.0)/CH3CN=40/60
Flow rate    : 0.5mL/min
Detector     : UV(223nm)
Column temp. : 40°C

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