One of short amine, atropine was analyzed using ODP2 HP without addition of ion pair reagent. ODP2 HP is reversed phase column packed polymer beads, and it can be used with alkali eluent. So there is no need to add ion pair reagent.

Please refer Analysis of Short Amines without Using Ion Pair Reagent to know detail about merit of using polymer based column.


Sample : Atropine (pKa9.9) 300 mg/L

Column       : Shodex ODP2 HP-4D (4.6 mm I.D. x 150 mm)
Eluent       : 10 mM Sodium phosphate buffer (pH11)/CH3CN=55/45
Flow rate    : 1.0 mL/min
Detector     : UV (220 nm)
Column temp. : 40 °C
Column       : ODS from other manufacturer (4.6 mm I.D. x 150 mm)
Eluent       : 0.1 % Sodium 1-pentanesulfonate aq./CH3CN=55/45
Flow rate    : 1.0 mL/min
Detector     : UV (220 nm)
Column temp. : 40 °C