Effects of pH of Eluent on Sensitivity of Barbital

Effects of eluent pH on sensitivity of barbital analysis using Asahipak ODP-50 4D (a column for reversed phase chromatography) is shown.The analysis with alkaline eluent(pH 11.0) gives the best result.

Sample : Barbital

Column       : Shodex Asahipak ODP-50 4D (4.6 mm I.D. x 150 mm)
Eluent       : 25 mM Sodium phosphate buffer/CH3CN=60/40
Flow rate    : 0.6 mL/min
Detector     : UV (254 nm)
Column temp. : 30 °C

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