Cinchona Alkaloids

For the analysis of cinchona alkaloids, cinchonine and quinine, which are basic drug components having tertiary amino groups, good separation can be obtained with alkaline eluent at pH9.0 using an Asahipak ODP-50 6D column. The separation at pH7.4 when using ODP or ODS columns is not as good as that obtained at pH9.0 when using an ODP column.

Sample :
1. Cinchonine , 2. Quinine

Column       : Shodex Asahipak ODP-50 6D, ODS from other manufacturer
               (6.0 mm I.D. x 150 mm each)
Eluent       : 10 mM Phosphate buffer/CH3CN/CH3OH=40/20/40
Flow rate    : 1.0 mL/min
Detector     : UV (250 nm)
Column temp. : 30 °C

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