Separation of Gingerol and Shogaol (C18M 4D)

Gingerol and shogaol are pungent component contained in ginger. It is known that they have the anti-oxidant, anti-thrombotic and anti-ulcer action. Shogaol hardly exists in fresh ginger. It is secondarily produced from gingerol when ginger is dried or heated. 6-gingerol and 6-shogaol were separated by Silica C18M 4D, a ODS column.

Sample: 10 μL
0.1 mg/mL each

  1. 1.6-Gingerol
  2. 2.6-Shogaol
Column
Shodex Silica C18M 4D (4.6 mm I.D. x 150 mm)
Eluent
(A); H2O (B); CH3CN
Linear gradient; (B %) 40 % to 70 % (15 min)
Flow rate
1.0 mL/min
Detector
UV (280 nm)
Column temp.
40 ℃

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