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Separation of Gingerol and Shogaol (C18M 4D)


Gingerol and shogaol are pungent component contained in ginger. It is known that they have the anti-oxidant, anti-thrombotic and anti-ulcer action. Shogaol hardly exists in fresh ginger. It is secondarily produced from gingerol when ginger is dried or heated. 6-gingerol and 6-shogaol were separated by Silica C18M 4D, a ODS column.

Sample : 0.1mg/mL each, 10μL
1. 6-Gingerol
2. 6-Shogaol


Column       : Shodex シリカ C18M 4D (4.6mmI.D. x 150mm)
Eluent       : (A) ; H2O (B) ; CH3CN
               Linear gradient : (B%) 40% to 70% (15min)
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : 40°C

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