Separation of Gingerol and Shogaol (C18M 4D)

Gingerol and shogaol are pungent component contained in ginger. It is known that they have the anti-oxidant, anti-thrombotic and anti-ulcer action. Shogaol hardly exists in fresh ginger. It is secondarily produced from gingerol when ginger is dried or heated. 6-gingerol and 6-shogaol were separated by Silica C18M 4D, a ODS column.

Sample : 10 μL
0.1 mg/mL each
1. 6-Gingerol
2. 6-Shogaol

Column       : Shodex Silica C18M 4D (4.6 mm I.D. x 150 mm)
Eluent       : (A) ; H2O (B) ; CH3CN
               Linear gradient :  40 B % to 70 B % (15 min)
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : 40 °C

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