SEC Analysis of Antibody Drug Conjugate (LW-803)

Antibody Drug Conjugates (ADCs) are a class of drugs where an antibody is linked to a small-molecule drug via a linker. ​This combines both the advantages of current small-molecule drugs as well as antibody drugs, and are attracting attention as the next-generation drug.
Here, an antibody drug conjugate formulation was analyzed using the aqueous SEC (GFC) column, PROTEIN LW-803. Besides monomers, a peak that appeared to be dimers was confirmed. The LW-803 is applicable for complex ADCs impurity analysis.

Sample : 20 µL
Antibody-drug conjugate
formulation 5 mg/mL
(in H2O)
1. Dimer
2. Monomer

Column       : Shodex PROTEIN LW-803 (8.0 mm I.D. x 300 mm)
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl/CH3CN=90/10
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : 25 °C 

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