Levocarnitine is a hydrophilic substance used for the treatment of primary carnitine deficiency.
Levocarnitine was analyzed using Asahipak NH2P-50 4E, a polymer-based amino column, under HILIC mode condition. It was found that an addition of salt in the eluent stabilizes the retention of levocarnitine. Also, by introducing 50 mM KH₂PO₄ aq. solution as a preconditioning solvent helps shortening the column equilibration time.

[Note] This analysis requires column equilibration.
For column equilibration: Introduce 50 mM KH₂PO₄ aq. at 0.5 mL/min flow rate for 2 hours.
Then, change the solvent with the eluent.


Sample : 1 %, 10 μL
1. Levocarnitine

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : 50 mM KH2PO4/CH3CN=35/65 (Adjusted with H3PO4 to a pH4.7)
Flow rate    : 1.0 mL/min
Detector     : UV (205 nm)
Column temp. : 30 °C