Sulfanilamides (DE-413)

Sulfanilamides were analyzed using RSpak DE-413, a column for reversed phase chromatography.


Sample : 20 mg/L each, 10 μL
1. Succinylsulfathiazole
2. Sulfadiazine
3. Sulfamerazine
4. Sulfathiazole
5. Sulfamethazine
6. Sulfanilamide


Column       : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent       : 25 mM Sodium phosphate buffer (pH7.6)/CH3CN/=82/18
Flow rate    : 1.0 mL/min
Detector     : UV (254 nm)
Column temp. : 40 °C

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