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Sulfanilamides (DE-413)

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Sulfanilamides were analyzed using RSpak DE-413, a column for reversed phase chromatography.


Sample : 20mg/L each, 10μL
1. Succinylsulfathiozole
2. Sulfadiazine
3. Sulfamerazine
4. Sulfathiazole
5. Sulfamethazine
6. Sulfanilamide


Column       : Shodex RSpak DE-413 (4.6mmI.D. x 150mm)
Eluent       : 25mM Sodium phosphate buffer(pH7.6)/CH3CN/=82/18
Flow rate    : 1.0mL/min
Detector     : UV(254nm)
Column temp. : 40°C

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