Streptomycin which is an aminoglycoside antibiotic, and its reduced form dihydrostreptomycin were analyzed using HILICpak VC-50 2D, a polymer-based HILIC mode column, with LC/MS detection. Generally, ODS columns are used for this type of analysis, but it takes time to stabilize the column due to its requirement of an ion pair reagent. The analysis using VC-50 2D does not require an ion pair reagent, allowing the antibiotics to be separated under cation exchange mode.
Sample : 20 μL
50 ng/mL each (in H2O)
Column : Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm) Eluent : 0.25 M HCOOH aq./CH3CN=90/10 Flow rate : 0.3 mL/min Detector : ESI-MS (SIM Positive) Column temp. : 40 °C
Sample Name Index
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