LC/MS Analysis of Streptomycins (VC-50 2D)

Streptomycin which is an aminoglycoside antibiotic, and its reduced form dihydrostreptomycin were analyzed using HILICpak VC-50 2D, a polymer-based HILIC mode column, with LC/MS detection. Generally, ODS columns are used for this type of analysis, but it takes time to stabilize the column due to its requirement of an ion pair reagent. The analysis using VC-50 2D does not require an ion pair reagent, allowing the antibiotics to be separated under cation exchange mode.

Sample: 20 μL
50 ng/mL each (in H2O)

  1. Streptomycin sulfate
  2. Dihydrostreptomycin sulfate
Column
Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm)
Eluent
0.25 M HCOOH aq./CH3CN=90/10
Flow rate
0.3 mL/min
Detector
ESI-MS (SIM Positive)
Column temp.
40 ℃

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