Fosfomycin  is one of the antibiotics with a relatively broad antibacterial spectrum. According to the monograph of fosfomycin tromethamine in the United States Pharmacopeia and the National Formulary (USP-NF 2021 Issue 1*), the thermal decomposition products of fosfomycin tromethamine (Solution A) and the standard-added fosfomycin (System suitability solution) are separated by HPLC. In this application, the targets were separated by Asahipak NH2P-50 4E.

*The version at the time of the application acquisition.

Preparation method: Solution A

1. (1)Mix 300 mg of fosfomycin tromethamine and 60 μL of water. Heat at 60 ° C for 24 hr.
2. (2)Add 20 mL of the eluent to (1).
3.  

Preparation method: System suitability solution

1. (1)Dissolve 120 mg of fosfomycin tromethamine in 1 mL of Solution A.
2.  

Sample: 5 μL
A: Solution A
B: System suitability solution

1. 1.Fosfomycin