Separation of Ceftazidime and Arginine (KW402.5-4F)

Ceftazidime is a cephem antibiotic used to treat bacterial infections. Some formulations of ceftazidime contain arginine. KW402.5-4F, an aqueous SEC (GFC) column, was used to separate ceftazidime and arginine, and a good separation was obtained.

Sample: 0.2 mg/mL each, 10 µL

  1. 1.Ceftazidime
  2. 2.L-Arginine
Ceftazidime_chromatogram
Column
Shodex KW402.5-4F (4.6 mm I.D. x 300 mm)
Eluent
1.15 g/L NH4H2PO4 (pH 2.0 adjusted with H3PO4)/CH3CN=25/75
Flow rate
0.5 mL/min
Detector
UV (206 nm)
Column temp.
30 ℃

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