SEC Analysis of Saccharated Ferric Oxide Injection (SB-804 HQ + SB-802.5 HQ)

Saccharated Ferric Oxide injection, a kind of intravenous iron injection, was analyzed with SEC (GFC) column OHpak SB-804 HQ and SB-802.5 HQ in series. It is possible to calculate the molecular weight of Saccharated Ferric Oxide. Shodex Standard P-82 (pullulan, molecular weight from 5,000 to 400,000) was used as a standard. It may be possible to use this analysis condition for Iron sucrose table as well. Both Saccharated Ferric Oxide and Iron sucrose have similar chemical structure.

Sample : 20 µL
2-fold diluted Saccharated ferric oxide injection with eluent
1. Saccharated ferric oxide

Columns      : Shodex OHpak SB-804 HQ + SB-802.5 HQ (8.0 mm I.D. × 300 mm each)
Eluent       : 7.16 g/L Na2HPO4・12H2O + 3.59 g/L NaH2PO4・2H2O aq.
Flow rate    : 0.5 mL/min
Detector     : RI
Column temp. : 45 °C

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