Analysis of Carboplatin (NH2P-50 4E)

Carboplatin, an anticancer drug, is called a platinum-based drug as it contains platinum in its structure. According to the United States Pharmacopeia and the National Formulary (USPNF 2022 Issue 1*), ASSAY of carboplatin should be carried out with a column filled with L8 packing material (aminopropylsilane chemically bonded to totally porous silica gel). Meanwhile, this application shows that a polymer-based amino column, Asahipak NH2P-50 4E is also feasible analyzing carboplatin following the similar conditions mentioned in the monogram.

(Note)
Asahipak NH2P-50 4E is a L82 column.

*The version at the time of the application acquisition.

Sample: 10 µL

  1. 1.Carboplatin 1 mg/mL
Carboplatin_chromatogram
Column
Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent
CH3CN/H2O=83/17
Flow rate
1.5 mL/min
Detector
UV (230 nm)
Column temp.
25 ℃

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