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LC/MS/MS Analysis of Ribavirin (VC-50 2D)


Ribavirin, a nucleic acid analog type therapeutic agent for hepatitis C, was analyzed by LC/MS/MS using a HILIC mode column HILICpak VC-50 2D. Hydrophilic compounds are retained at a high concentration of acetonitrile under HILIC mode conditions. This is advantageous for high sensitivity analysis by ESI-MS/MS.

Sample : 5μL
1. Ribavirin 0.5ng/mL
(in H2O/CH3CN=1/4)

Column       : Shodex HILICpak VC-50 2D (2.0mmI.D. x 150mm)
Eluent       : 50mM HCOOH aq./CH3CN=10/90
Flow rate    : 0.25mL/min
Detector     : ESI-MS/MS (MRM Positive)
Column temp. : 40°C

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