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Tetracycline Derivative Antibiotics

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Three kinds of tetracycline derivative antibiotics were analyzed using Asahipak ODP-40 4D (a column for reversed phase chromatography).


Sample : 0.1ppm each, 20μL
1. Oxytetracycline
2. Tetracycline
3. Chlortetracycline


Column       : Shodex Asahipak ODP-40 4D (4.6mmI.D. x 150mm)
Eluent       : Imidazole buffer/Methanol
Detdctor     : Fluorescence(Ex.380nm, Em.520nm)
Column temp. : 40°C

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