Tetracycline Derivative Antibiotics (ODP-40 4D)

Three kinds of tetracycline derivative antibiotics were analyzed using Asahipak ODP-40 4D (a column for reversed phase chromatography).
*Sales of Asahipak ODP-40 4D used in this application is discontinued. Please consider trying Asahipak ODP-50 4D.

Sample: 20 μL
0.1 μg/mL each

  1. 1.Oxytetracycline
  2. 2.Tetracycline
  3. 3.Chlortetracycline
Column
Shodex Asahipak ODP-40 4D (4.6 mm I.D. x 150 mm)
Eluent
Imidazole buffer/Methanol
Detector
Fluorescence (Ex. 380 nm, Em. 520 nm)
Column temp.
40 ℃

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