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Basic Drugs : Under Neutral Condition

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Elution patterns of four kinds of basic drugs which have tertiary amine are shown. Under the eluent conditions, large peak tailing and delay of elution are observed for ODS-A and ODS-B except scopolamine. To the contrary, the elution patterns of ODS-C (polymer-coated) and ODP (polymer-based) for procainamide and atropine are far better than those of ODS-A and ODS-B though peak tailing is observed for diphenhydramine. These chromatograms show non-specific adsorption which is usually observed for Asahipak ODP columns and its solution using polymer-based or polymer-coated columns.


Sample :
1.0mg/mL Scopolamine hydrochloride, 50μL
1.0mg/mLProcainamide hydrochloride, 40μL
0.5mg/mLDiphenhydramine hydrochloride, 2μL
1.0mg/mLAtropine , 50μL


Columns      : Shodex Asahipak ODP-50 4D (4.6mmI.D. x 150mm),
               ODS-A, ODS-B, ODS-C (4.6mmI.D. x 150mm each)
Eluent       : 25mM Phosphate buffer(pH7)/CH3CN=60/40
Flow rate    : 0.6mL/min
Detector     : UV(254nm)
Column temp. : 30°C

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