Elution patterns of four kinds of basic drugs which have tertiary amine are shown. Under the eluent conditions, large peak tailing and delay of elution are observed for ODS-A and ODS-B except scopolamine. To the contrary, the elution patterns of ODS-C (polymer-coated) and ODP (polymer-based) for procainamide and atropine are far better than those of ODS-A and ODS-B though peak tailing is observed for diphenhydramine. These chromatograms show non-specific adsorption which is usually observed for Asahipak ODP columns and its solution using polymer-based or polymer-coated columns.
1.0 mg/mL Scopolamine hydrochloride, 50 μL
1.0 mg/mLProcainamide hydrochloride, 40 μL
0.5 mg/mLDiphenhydramine hydrochloride, 2 μL
1.0 mg/mLAtropine , 50 μL
Columns : Shodex Asahipak ODP-50 4D (4.6 mm I.D. x 150 mm), ODS-A, ODS-B, ODS-C (4.6 mm I.D. x 150 mm each) Eluent : 25 mM Phosphate buffer (pH7)/CH3CN=60/40 Flow rate : 0.6 mL/min Detector : UV (254 nm) Column temp. : 30 °C
Sample Name Index
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