This figure shows the chromatogram of protein mixtures analyzed with OHpak SB-803 HQ, Asahipak GF-510 HQ or PROTEIN KW-803. KW-803 is the best column for the separetion of these proteins.
Sample :
A: Thyroglobulin (bovine)
B: γ-Globulin (bovine)
C: Ovalbumin (chiken)
D: Myoglobin (horse)
E: Cyanocobalamin
Columns : Shodex OHpak SB-803 HQ (8.0 mm I.D. x 300 mm) Shodex Asahipak GF-510 HQ (7.5 mm I.D. x 300 mm) Shodex PROTEIN KW-803 (8.0 mm I.D. x 300 mm) Eluent : 0.2 M Phosphate buffer (pH6.9) Flow rate : 0.5 mL/min Detector : UV (280 nm) Column temp. : 30 °C
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