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Comparison of Chromatograms of Protein mixtures


This figure shows the chromatogram of protein mixtures analyzed with OHpak SB-803 HQ, Asahipak GF-510 HQ or PROTEIN KW-803. KW-803 is the best column for the separetion of these proteins.

Sample :
A: Thyroglobulin (bovine)
B: γ-Globulin (bovine)
C: Ovalbumin (chiken)
D: Myoglobin (horse)
E: Cyanocobalamin

Columns      : Shodex OHpak SB-803 HQ (8.0 mm I.D. x 300 mm) 
               Shodex Asahipak GF-510 HQ (7.5 mm I.D. x 300 mm)
               Shodex PROTEIN KW-803 (8.0 mm I.D. x 300 mm)
Eluent       : 0.2 M Phosphate buffer (pH6.9)
Flow rate    : 0.5 mL/min
Detector     : UV (280 nm)
Column temp. : 30 °C

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