Recovery of Proteins (GF-310 HQ and GS-320 HQ)

Asahipak GF-310 HQ, GS-320 HQ permit GFC analysis with good recovery of proteins.

Proteins	Recovery(%)
Proteins	GF-310 HQ	GS-320 HQ
Ovalbumin	95	93
Myoglobin	93	96
Lysozyme	95	86
Cytochrome c	96	93

Columns      : Shodex Asahipak GF-310 HQ, GS-320 HQ (7.5mmI.D. x 300mm each)
Eluent       : 0.1M Sodium phosphate buffer(pH7.0) + 0.1M NaCl
Flow rate    : 1.0mL/min
Detector     : UV(210nm)
Column temp. : 30°C

Sample name index

Cytochrome c
Lysozyme
Myoglobin
Ovalbumin

Product name index

GF-310 HQ(Asahipak)
GS-320 HQ(Asahipak)

Applications

Sample Load (KW-803)
Effects of Flow Rate (KW-802.5, KW-803)
Standard Proteins (1) (KW-802.5)
Standard Proteins (2) (KW-802.5 and KW-803)
Standard Proteins (14) (KW-802.5)
Standard Proteins (15) (KW-803)
Standard Proteins (16) (KW-804)
Standard Proteins (18) (KW-803)
Standard Proteins (19) (KW-804)
Standard Proteins (23) (GF-510 HQ)
Standard Proteins (24) (KW402.5-4F)
Standard Proteins (25) (KW403-4F)
Standard Proteins (26) (KW404-4F)
Standard Proteins (27) (KW405-4F)
Recovery of Proteins (KW-802.5 and KW-803)
Recovery of Proteins (KW-803)
Proteins in Human Serum (3) (KW-803)
Dimer and Trimer of BSA
Crude Albumin (Chicken Egg) (1) (KW-803)
Crude Lipoxidase
Crude Catalase
Crude Hexokinase
Human Serum (1) (SB-802 HQ)
Effects of Injection Volume of Serum
Tryptic Digest of Human IgG
Comparison of Chromatograms of Protein mixtures
Comparison between KW402.5-4F and KW-802.5
Recovery of Proteins (KW402.5-4F, KW403-4F)
Human Control Serum
Analysis of Whey in Yogurt (KW403 4F + KW402.5 4F)
Lectin Protein
Peptide Analysis (SEC)
PEG-Chymotrypsin (KW-802.5)
Human IgM (2)
Poly-Lysine
Avidin (2)
α₁ Acid Glycoprotein
Analysis of Gelatin (SB-806M HQ)
Analysis of Insulin Glargine According to USP Method (KW-802.5)
Analysis of Insulin Glargine According to JP Method (KW-802.5)
Analysis of Human Insulin According to JP Method (KW-802.5)
Analysis of High Molecular Impurities in Insulin Aspart According to Pharmacopeia Method (KW-802.5)
Analysis of Insulin According to USP Method (KW-802.5)
Comparison of SEC Separation of Standard Proteins
Comparison of SEC Separation of Polyclonal Antibody
Comparison of SEC Separation of Monoclonal Antibody
Comparison of Separation of BSA Aggregates
Monitoring Papain Digestion of Humanized Monoclonal IgG (LW-803)
Analysis of Lipoproteins in Serum (KW-804)
Comparison of SEC Separation of Standard Proteins between LW-403 4D and LW-803
Comparison of SEC Separation of IgG between LW-403 4D and LW-803
Durability Test by Number of Sample Injections (LW-403 4D)
Analysis of Epoetin According to USP Method (LW-803)
Analysis of Hemocyanin (KW405-4F)
Comparison of Separation of Proteins with Conventional Device and Semi-micro Type Device (LW-403 4D)
Comparison of IgG Separation with Conventional Device and Semi-micro Type Device (LW-403 4D)
Particle Size Distribution Determination of Hepatitis B Surface Antigen Virus-like Particle (HBsAg VLP) (KW405-4F)
Analysis of Somatropin According to EP Method (LW-803)
SEC Analysis of Rituximab (LW-803)
SEC Analysis of PEG-Asparaginase (SB-806 HQ)
SEC/MALS Analysis of Norovirus VLP (SB-805 HQ)
Analysis of Thymosin α1 (KW-802.5)
SEC/MALS Analysis of Exosome (SB-806 HQ)