The durability test for PROTEIN LW-403 4D, an aqueous SEC (GFC) semi-micro column for rapid analysis of antibody proteins, by the number of sample injections was conducted. It is confirmed that the column performance has barely changed after 500 injections.


Sample : 0.5 μL
IgG from human serum 10 mg/mL
(D) Dimer
(M) Monomer

Column       : Shodex PROTEIN LW-403 4D (4.6 mm I.D. x 150 mm)
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : 0.35 mL/min
Detector     : UV (280 nm) (small cell volume)
Column temp. : Room temp.