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Durability Test by Number of Sample Injections (LW-403 4D)


The durability test for PROTEIN LW-403 4D, an aqueous SEC (GFC) semi-micro column for rapid analysis of antibody proteins, by the number of sample injections was conducted. It is confirmed that the column performance has barely changed after 500 injections.

Sample : 0.5μL
IgG from human serum 10mg/mL
(D) Dimer
(M) Monomer

Column       : Shodex PROTEIN LW-403 4D (4.6mmI.D. x 150mm)
Eluent       : 50mM Sodium phosphate buffer (pH7.0) + 0.3M NaCl
Flow rate    : 0.35mL/min
Detector     : UV(280nm) (small cell volume)
Column temp. : Room temp.

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