Analysis of Gelatin (SB-806M HQ)

Gelatin is a purified protein which is made by partial acid hydrolysis or partial alkaline hydrolysis of animal collagen, and is used for stabilizer, lubricant, binder, brightening agent, or courting agent. In this application, two kinds of gelatin from animals are shown.


Sample : 0.1 % each, 100 µL
Gelatin from bovine skin
(Acid treatment, Gel strength : 225 g)
Gelatin from porcine skin
(Alkali treatment, Gel strength : 90-100 g)

Columns      : Shodex OHpak SB-806M HQ (8.0 mm I.D. x 300 mm) x 2
Eluent       : 0.1 M KH2PO4 aq./0.1 M Na2HPO4 aq.=50/50
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 40 °C