Effects of flow rate when analyzing proteins using PROTEIN KW-802.5 and KW-803 (columns for aqueous SEC (GFC)) were shown.
Sample : BSA, Myoglobin, 0.2 % each, 50 μL
Columns : (Left); Shodex PROTEIN KW-802.5, (Right); KW-803 (8.0 mm I.D. x 300 mm each) Eluent : 50 mM Phosphate buffer + 150 mM Na2SO4 Flow rate : 1.0 mL/min Detector : UV (280 nm) Column temp. : 25 °C
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