Whole lipoproteins from serum of a healthy person were analyzed using PROTEIN KW-804, an aqueous SEC (GFC) column. The result shows a good separation of the three kinds of lipoproteins (VLDL, LDL and HDL) in serum.

(Preparation of sample)
- Adjust specific gravity of serum of a healthy person to 1.210 g/mL added potassium bromide followed by ultracentrifugation for 24 hours.
- After dialyzing the supernatant, solvent substitute by PBS^*.
- Measure protein concentration by Lowry method and dilute with PBS* to 1.0 mg/mL.


Sample : 40 µL
Whole lipoproteins from serum of a healthy person 1.0 mg/mL
1. VLDL
2. LDL
3. HDL

Columns      : Shodex PROTEIN KW-G 6B (6.0 mm I.D. x 50 mm) + KW-804 (8.0 mm I.D. x 300 mm)
Eluent       : 10-fold diluted x10 PBS* with H2O
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : 30 °C

x10 PBS* : 80 g NaCl + 29 g Na2HPO4・12H2O + 2 g KCl + 2 g KH2PO4 in 1000 mL of H2O

Data courtesy of Ohkawa Ryunosuke,
Graduate School of Health Care Sciences, Analytical Laboratory Chemistry, Tokyo Medical and Dental University