Analysis of Lipoproteins in Serum (KW-804)

Whole lipoproteins from serum of a healthy person were analyzed using PROTEIN KW-804, an aqueous SEC (GFC) column. The result shows a good separation of the three kinds of lipoproteins (VLDL, LDL and HDL) in serum.

Sample preparation

  • Adjust specific gravity of serum of a healthy person to 1.210 g/mL added potassium bromide followed by ultracentrifugation for 24 hours.
  • After dialyzing the supernatant, solvent substitute by PBS*.
  • Measure protein concentration by Lowry method and dilute with PBS* to 1.0 mg/mL.

Sample: 40 μL
Whole lipoproteins from serum of a healthy person 1.0 mg/mL

  1. 1.VLDL
  2. 2.LDL
  3. 3.HDL
Column
：Shodex PROTEIN KW-G 6B (6.0 mm I.D. x 50 mm) + KW-804 (8.0 mm I.D. x 300 mm)
Eluent
：10-fold diluted x10 PBS* with H2O
(x10 PBS*: 80 g NaCl + 29 g Na2HPO4・12H2O + 2 g KCl + 2 g KH2PO4 in 1000 mL of H2O)
Flow rate
：1.0 mL/min
Detector
：UV (280 nm)
Column temp.
：30 ℃

Data courtesy of Prof. Ohkawa Ryunosuke, Graduate School of Health Care Sciences, Analytical Laboratory Chemistry, Tokyo Medical and Dental University

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