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Sample Load (KW-803)

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Shodex PROTEIN KW-803 (a column for aqueous SEC (GFC)) shows good efficiency until 0.5 mg of BSA (Bovine serum albumin) injection. If the injection to KW-803 exceeds 1 mg, chromatogram of BSA becomes gradually broad. Recommended sample loading is less than 0.5 mg of BSA per column.


Sample : BSA, 100 μL


Column       : Shodex PROTEIN KW-803 (8.0 mm I.D. x 300 mm)
Eluent       : 50 mM Phosphate buffer (pH7.0) + 150 mM NaCl
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : Room temp.

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