The outside structure of a virus-like particle (VLP) mimics a virus, but it does not contain viral genetic material. Thus, it is considered as a safe empty particle, and is widely used for the production of vaccines or development of drug delivery system in pathology and immunology. VLPs are often produced by microbial expression system.
In this application, a crude extract of norovirus VLP (NVLP) was analyzed by a SEC-MALS method. The crude extract was purified by an anion exchange column, CIM multusTM QA-1 . For the separation of impurities and the NVLP was achieved by using a polymer-base aqueous SEC (GFC) column, OHpak SB-805 HQ. Since UV detector cannot distinguish the impurities and the NVLP, the use of an appropriate SEC column is important. The obtained rms radius of the extract reflected the size distribution of the properly configured NVLP particle size.
Because of its sophisticated high hydrophilicity design, SB-805 HQ experiences less adsorption and have the pore size appropriate for the analysis of nanoparticles. Therefore, it is a suitable and a recommended separation tool for VLP profiling.
Please refer to ShodexTM Technical Article No.15 "Purification and SEC/MS Analysis of Norovirus Virus-Like Particles"
Sample : 50 μL
NVLP purification Fraction
of an anion exchange chromatography
Column : Shodex OHpak SB-805 HQ (8.0 mm I.D. x 300 mm) Eluent : PBS (-) (pH7.4) Flow rate : 1.0 mL/min Detector : UV (280 nm) (small cell volume) MALS (DAWN8+ produced by Wyatt Technology Corp.) Column temp. : 25 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Sample Load (KW-803)
- Effects of Flow Rate (KW-802.5, KW-803)
- Standard Proteins (1) (KW-802.5)
- Standard Proteins (2) (KW-802.5 and KW-803)
- Standard Proteins (14) (KW-802.5)
- Standard Proteins (15) (KW-803)
- Standard Proteins (16) (KW-804)
- Standard Proteins (18) (KW-803)
- Standard Proteins (19) (KW-804)
- Standard Proteins (23) (GF-510 HQ)
- Standard Proteins (24) (KW402.5-4F)
- Standard Proteins (25) (KW403-4F)
- Standard Proteins (26) (KW404-4F)
- Standard Proteins (27) (KW405-4F)
- Recovery of Proteins (GF-310 HQ and GS-320 HQ)
- Recovery of Proteins (KW-802.5 and KW-803)
- Recovery of Proteins (KW-803)
- Proteins in Human Serum (3) (KW-803)
- Dimer and Trimer of BSA
- Crude Albumin (Chicken Egg) (1) (KW-803)
- Crude Lipoxidase
- Crude Catalase
- Crude Hexokinase
- Human Serum (1) (SB-802 HQ)
- Tryptic Digest of Human IgG
- Comparison of Chromatograms of Protein mixtures
- Comparison between KW402.5-4F and KW-802.5
- Recovery of Proteins (KW402.5-4F, KW403-4F)
- Human Control Serum
- Analysis of Whey in Yogurt (KW403 4F + KW402.5 4F)
- Lectin Protein
- Peptide Analysis (SEC)
- PEG-Chymotrypsin (KW-802.5)
- SEC Analysis of PEG-Asparaginase (SB-806 HQ)
- Human IgM (2)
- Poly-Lysine
- Avidin (2)
- α1 Acid Glycoprotein
- Analysis of Gelatin (SB-806M HQ)
- Analysis of Insulin Glargine According to JP Method (KW-802.5)
- Analysis of Human Insulin According to JP Method (KW-802.5)
- Analysis of High Molecular Impurities in Insulin Aspart According to Pharmacopeia Method (KW-802.5)
- Analysis of High Molecular Weight Protein According to USP-NF GENERAL CHAPTERS Physicochemical Analytical Procedures for Insulins (KW-802.5)
- Comparison of SEC Separation of Standard Proteins
- Comparison of SEC Separation of Polyclonal Antibody
- Comparison of SEC Separation of Monoclonal Antibody
- Comparison of Separation of BSA Aggregates (LW-803, KW-803)
- Monitoring Papain Digestion of Humanized Monoclonal IgG (LW-803)
- Analysis of Lipoproteins in Serum (KW-804)
- Comparison of SEC Separation of Standard Proteins between LW-403 4D and LW-803
- Durability Test by Number of Sample Injections (LW-403 4D)
- Analysis of Epoetin According to USP-NF Method (LW-803)
- Comparison of Separation of Proteins with Conventional Device and Semi-micro Type Device (LW-403 4D)
- Comparison of IgG Separation with Conventional Device and Semi-micro Type Device (LW-403 4D)
- Particle Size Distribution Determination of Hepatitis B Surface Antigen Virus-like Particle (HBsAg VLP) (KW405-4F)
- Analysis of Hemocyanin (KW405-4F)
- Analysis of Somatropin According to EP Method (LW-803)
- SEC Analysis of Rituximab (LW-803)
- Analysis of Thymosin α1 (KW-802.5)
- SEC/MALS Analysis of Exosome (SB-806 HQ)
- Analysis of Proteoglycan in a Supplement (SB-806M HQ)
- Molecular Weight Measurements of Low Molecular Weight Heparins According to USP-NF GENERAL CHAPTERS <209> (KW-803 + KW-802.5)
Applications (Related Information)
- Calibration Curves for KW-800 Series (Pullulan)
- Calibration Curves for KW-800 Series (Proteins)
- Calibration Curves for KW-800 Series (PEG, PEO)
- Calibration Curves of KW400-4F (1) (Pullulan)
- Calibration Curves of KW400-4F (2) (Proteins)
- Calibration Curves of KW400-4F (3) (PEG, PEO)
- Calibration Curves for GF-HQ Series (Aqueous Eluent: Proteins)
- Calibration Curve for LW-803 (Proteins)
- Calibration Curve for LW-403 4D (Proteins)