This figure shows the separation of standard proteins using aqueous SEC (GFC) mode. Two columns for GFC separation, PROTEIN KW-802.5 and KW-803 were used. KW-802.5 is suitable for the analysis of proteins with molecular weights of less than 150,000, while KW-803, which has a larger pore size than KW-802.5, is suitable for the analysis of proteins with molecular weights of more than 150,000.


Sample : Molecular weight marker
1. Glutamate dehydrogenase
2. Lactate dehydrogenase
3. Enolase
4. Adenylate kinase
5. Cytochrome c

Columns      : Shodex PROTEIN KW-802.5, KW-803 (8.0 mm I.D. x 300 mm each)
Eluent       : 50 mM Phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : Room temp.