Standard Proteins (24) (KW402.5-4F)

Ten kinds of protein standards were analyzed using KW402.5-4F (a semi-micro column for aqueous SEC (GFC)).



Sample : 5 μL
1. Thyroglobulin (MW: 669,000)
2. γ-Globulin (MW: 158,000)
3. BSA (MW: 67,000)
4. Ovalbumin (MW: 43,000)
5. Peroxidase (MW: 40,200)
6. β-Lactoglobulin (MW: 35,000)
7. Myoglobin (MW: 17,000)
8. Ribonuclease A (MW: 13,700)
9. Aprotinin (MW: 6,500)
10. Vitamin B₁₂ (MW: 1,350)

Column       : Shodex KW402.5-4F (4.6 mm I.D. x 300 mm)
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : 0.33 mL/min
Detector     : UV (220 nm) (small cell volume)
Column temp. : 30 °C