Protein analysis data using KW402.5-4F, high performance semi-micro aqueous SEC (GFC) column, and current PROTEIN KW-802.5. Theoretical plate number of KW400-4F series has improved 1.5 times than KW-800 series. Sensitivity becomes 3 or 4 times better than current column. KW400-4F series is suitable for high resolution and high sensitivity analysis.


Sample : 10 μL
1. Blue dextran 2000, 0.2 mg/mL
2. γ-Globulin, 0.8 mg/mL
3. Ovalbumin, 0.8 mg/mL
4. Myoglobin, 0.56 mg/mL
5. Uridine, 0.04 mg/mL

Columns      : (A); Shodex KW402.5-4F (4.6 mm I.D. x 300 mm)
               (B); Shodex PROTEIN KW-802.5 (8.0 mm I.D. x 300 mm)
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : (A); 0.33 mL/min, (B); 1.0 mL/min
Detector     : UV (280 nm) (small cell volume)
Column temp. : Room temp.(25 °C)