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Human Control Serum

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Human control serum was analyzed using aqueous solvent SEC (GFC) high performance semi-micro columns, KW403-4F and KW404-4F. The bigger pore size (1,500A compared to 800A) of packed gel in KW404-4F is suitable for the analysis of large molecules that were excluded as void when using a KW403-4F column.



Sample : 1μL
Control serum I wako/B


Columns      : Shodex KW403-4F, KW404-4F (4.6mmI.D. x 300mm each)
Eluent       : 50mM Sodium phosphate buffer (pH7.0) + 0.3M NaCl 
Flow rate    : 0.20mL/min
Detector     : UV(280nm) 
Column temp. : 25°C

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