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Analysis of Hemocyanin (KW405-4F)


Hemocyanin is a respiratory pigment present in body fluids of mollusks and crustaceans, and is macro protein association composed of ten (or more) subunits which have the molecular weight on the several hundred kDa scale. In this application, Hemocyanin from keyhole limpet was analyzed using KW405-4F, an aqueous SEC (GFC) column. The estimated exclusion limit of KW405-4F is 20,000, 000 Da, and it is suitable for the analysis of macro-proteins.

Sample : 10 μL
(from keyhole limpet) 16 mg/mL

Column       : Shodex KW405-4F (4.6 mm I.D. x 300 mm)
Eluent       : PBS (-) (pH7.4) 
Flow rate    : 0.35 mL/min
Detector     : UV (280 nm) (small cell volume) 
               MALS (µDAWN produced by Wyatt Technology Corp.)
Column temp. : 25 °C

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