Analysis of Thymosin α1 (KW-802.5)

Thymosin α1 is a biologically active polypeptide isolated from thymus. It is known to have immunomodulatory, antiviral, angiogenic, and wound healing effects, and has been reported to activate tumor-related macrophages. In China, subcutaneous injection of thymosin α1 is suggested to improve immunity which is considered to prevent the aggravation of coronavirus-infected persons.
By using an aqueous SEC (GFC) column, PROTEIN KW-802.5, each component in a sample containing ribonuclease, human insulin, thymosin α1 and somatostatin were separated well and they eluted in descending order of molecular weight.

Sample : 1 mg/mL each, 5 μL
1. Ribonuclease A (MW: 13,700)
2. Human insulin (MW: 5,808)
3. Thymosin α1 (MW: 3,108)
4. Somatostatin (MW: 1,521)

Column       : Shodex PROTEIN KW-802.5 (8.0 mm I.D. x 300 mm)
Eluent       : 0.1 %(w/v) L-Arginine aq./CH3CN=550/450, pH 3.0 adjusted with TFA
Flow rate    : 1.0 mL/min
Detector     : UV (220 nm) 
Column temp. : 25 °C

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