Calibration curves for KW400-4F series, columns for aqueous SEC (GFC), using proteins are shown. KW405-4F was designed to have a larger pore size than KW-804. KW405-4F can analyze high molecular weight proteins which have several millions or more molecular weight such as agglomerated proteins.
Sample :
Thyroglobulin (MW : 670,000)
γ-Globulin (MW : 160,000)
BSA (MW : 69,000)
Ovalbumin (MW : 45,000)
Adenylate kinase (MW : 32,000)
Myoglobin (MW : 17,000)
Ribonuclease A (MW : 13,000)
Aprotinin (MW : 6,500)
Columns : Shodex KW400-4F series (4.6 mm I.D. x 300 mm each) Eluent : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl Flow rate : 0.33 mL/min Detector : UV (280 nm) Column temp. : 30 °C
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