Calibration Curves of KW400-4F (2) (Proteins)

Calibration curves for KW400-4F series, columns for aqueous SEC (GFC), using proteins are shown. KW405-4F was designed to have a larger pore size than KW-804. KW405-4F can analyze high molecular weight proteins which have several millions or more molecular weight such as agglomerated proteins.


Sample :
Thyroglobulin (MW : 670,000)
γ-Globulin (MW : 160,000)
BSA (MW : 69,000)
Ovalbumin (MW : 45,000)
Adenylate kinase (MW : 32,000)
Myoglobin (MW : 17,000)
Ribonuclease A (MW : 13,000)
Aprotinin (MW : 6,500)

Columns      : Shodex KW400-4F series (4.6 mm I.D. x 300 mm each)
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : 0.33 mL/min
Detector     : UV (280 nm)
Column temp. : 30 °C