Collagen is a type of protein that forms a triple helix structure, and gelatin unravels this triple helix structure through heat treatment. Collagen peptides are the breakdowns of gelatin. Since small molecules are easily absorbed by body, ingestion of collagen prptides is more efficient way of taking collagen. Thus, use of collagen peptide has been attracting people’s interests. Edible collagen peptides are standardized by the “Gelatin Manufacturers Association of Japan (GMJ)”. As a standard test method, the GMJ method is recommended for molecular weight distribution measurement. This method is specialized for measuring molecular weight distribution of low-molecular-weight peptides rather than collagen or gelatin. A silica-based aqueous SEC (GFC) column PROTEIN KW-802.5 is suitable for analysis of collagen peptides in the target molecular weight range. The calibration curve obtained showed a high linearity with a coefficient of determination R²=0.9989. These makes PROTEIN KW-802.5 suitable for the GMJ method. In this application, a soft drink containing collagen peptides was analyzed using KW-802.5.

Sample: 10 μL
 Soft drink containing collagen peptides

1.  

Standard peptides 0.5 % each

1. 1.Cytochrome C (WM 12,384)
2. 2.Aprotinin (MW 6,512)
3. 3.Bacitracin (MW 1,423)
4. 4.Gly-Gly-Tyr-Arg (MW 451)
5. 5.Gly-Gly-Gly (MW 189)