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Comparison of IgG Separation with Conventional Device and Semi-micro Type Device (LW-403 4D)

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PROTEIN LW-403 4D is a rapid semi-micro column for aqueous SEC (GFC) suitable for antibody protein analysis. A comparison of IgG aggregate separation with a conventional device and a semi-micro type device was completed. The resolution of the conventional device is reduced due to the diffusion of the sample outside the column. We recommend using a semi-micro type device to achieve the best column performance.


Sample : 5 μL
IgG from human serum 2 mg/mL
1. Aggregates
2. Trimer
3. Dimer
4. Monomer


Semi-micro typeConventional type
Tubing 0.1 mm I.D. 0.25 mm I.D.
 UV detectorCell volume 2.5 μL 18 μL
Optical path length 5 mm 10 mm
Column       : Shodex PROTEIN LW-403 4D (4.6 mm I.D. x 150 mm)
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : 0.35 mL/min
Detector     : UV (280 nm)
Column temp. : Room temp.

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