PROTEIN LW-403 4D is an aqueous SEC (GFC) semi-micro column for rapid analysis of antibody proteins. PROTEIN LW-403 4D and PROTEIN LW-803 (a standard column) were compared for the separation of standard proteins. Analysis using LW-403 4D can be completed in half the time compared to LW-803.


Sample :
(LW-403 4D) 0.5 μL
(LW-803) 5 μL
1. Thyroglobulin
(MW : 670,000) 7 mg/mL
2. γ-Globulin
(MW : 160,000) 6 mg/mL
3. Ovalbumin
(MW : 44,300) 4.8 mg/mL
4. Ribonuclease A
(MW : 13,700) 7 mg/mL
5. Uridine
(MW : 244) 0.1 mg/mL

Columns      : Shodex PROTEIN LW-403 4D (4.6 mm I.D. x 150 mm)
               Shodex PROTEIN LW-803 (8.0 mm I.D. x 300 mm)
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : (LW-403 4D) 0.35 mL/min
               (LW-803) 1.0 mL/min
Detector     : (LW-403 4D) UV (280 nm) (small cell volume)
               (LW-803) UV (280 nm) (conventional type)
Column temp. : Room temp.