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Comparison of SEC Separation of Standard Proteins between LW-403 4D and LW-803

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PROTEIN LW-403 4D is an aqueous SEC (GFC) semi-micro column for rapid analysis of antibody proteins. PROTEIN LW-403 4D and PROTEIN LW-803 (a standard column) were compared for the separation of standard proteins. Analysis using LW-403 4D can be completed in half the time compared to LW-803.


Sample :
(LW-403 4D) 0.5μL
(LW-803) 5μL
1. Thyroglobulin
(MW : 670,000) 7mg/mL
2. γ-Globulin
(MW : 160,000) 6mg/mL
3. Ovalbumin
(MW : 44,300) 4.8mg/mL
4. Ribonuclease A
(MW : 13,700) 7mg/mL
5. Uridine
(MW : 244) 0.1mg/mL


Columns      : Shodex PROTEIN LW-403 4D (4.6mmI.D. x 150mm)
               Shodex PROTEIN LW-803 (8.0mmI.D. x 300mm)
Eluent       : 50mM Sodium phosphate buffer (pH7.0) + 0.3M NaCl
Flow rate    : (LW-403 4D) 0.35mL/min
               (LW-803) 1.0mL/min
Detector     : (LW-403 4D) UV(280nm) (small cell volume)
               (LW-803) UV(280nm) (conventional type)
Column temp. : Room temp.

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