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Calibration Curve for LW-403 4D (Proteins)

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A calibration curve for PROTEIN LW-403 4D, an aqueous SEC (GFC) semi-micro column for rapid analysis of antibody proteins, using proteins is shown.


Sample :
Thyroglobulin (MW : 670,000)
γ-Globulin (MW : 160,000)
BSA (MW : 66,500)
Ovalbumin (MW : 45,000)
Myoglobin (MW : 17,000)
Ribonuclease A (MW : 13,000)
Aprotinin (MW : 6,500)


Column       : Shodex PROTEIN LW-403 4D (4.6mmI.D. x 150mm)
Eluent       : 50mM Sodium phosphate buffer (pH7.0) + 0.3M NaCl
Flow rate    : 0.35mL/min
Detector     : UV(280nm) (small cell volume)
Column temp. : Room temp.

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