Tryptic Digest of Human IgG

When a tryptic digest of human lgG was analyzed using PROTEIN KW-803 and KW-802.5 (columns for Aqueous SEC (GFC)) with a UV detector (230 nm) as well as with an optical rotation detector (ORD). The ORD shows a negative peak and it gives the information that human lgG is levorotatory.


Sample : Triptic digest of human IgG


Columns      : Shodex PROTEIN KW-803 + KW-802.5 (8.0 mm I.D. x 300 mm each)
Eluent       : 0.05 M Tris-HCl buffer (pH7.6) + 0.15 M NaCl + 0.3 mM CaCl2
Flow rate    : 0.5 mL/min
Detector     : UV (230 nm), ORD
Column temp. : Room temp.

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