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Standard Proteins (18) (KW-803)

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When a sample contains high molecular weight portions, PROTEIN KW-803 (a column for aqueous SEC (GFC)) will give a better result than Company A column.



Sample : Gel Filtration Standard (Bio Rad)

Peak Protein PROTEIN KW-803 Company A column
Rt(min) Width(min) Rs Rt(min) Width(min) Rs
1 Thyroglobulin 6.5657 0.7550
6.5657 0.7550

(MW: 670,000)

2.59

2.27
2 γ-Globulin 8.2957 0.5825
7.7203 0.8450

(MW: 158,000)

2.33

1.84
3 Ovalbumin 9.5547 0.5000
8.9840 0.5250

(MW: 44,000)

1.93

2.39
4 Myoglobin 10.4380 0.4175
10.1290 0.4350

(MW: 17,000)

4.69

3.57
5 Vitamin B12 12.3423 0.3950
11.5647 0.3700

(MW: 1,350)






Columns      : (Left); Shodex PROTEIN KW-803 (8.0mmI.D. x 300mm),
               (Right); Company A column
Eluent       : 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate    : 1.0mL/min
Detector     : UV(220nm)
Column temp. : Room temp.

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