• HOME
  • Product Search
  • Application Search
  • Distributors
  • Support

Standard Proteins (18) (KW-803)

Print

When a sample contains high molecular weight portions, PROTEIN KW-803 (a column for aqueous SEC (GFC)) will give a better result than Company A column.



Sample : Gel Filtration Standard (Bio Rad)

PeakProteinPROTEIN KW-803Company A column
Rt(min)Width(min)RsRt(min)Width(min)Rs
1 Thyroglobulin 6.5657 0.7550 6.5657 0.7550
(MW: 670,000) 2.59 2.27
2 γ-Globulin 8.2957 0.5825 7.7203 0.8450
(MW: 158,000) 2.33 1.84
3 Ovalbumin 9.5547 0.5000 8.9840 0.5250
(MW: 44,000) 1.93 2.39
4 Myoglobin 10.4380 0.4175 10.1290 0.4350
(MW: 17,000) 4.69 3.57
5 Vitamin B12 12.3423 0.3950 11.5647 0.3700
(MW: 1,350)

Columns      : (Left); Shodex PROTEIN KW-803 (8.0 mm I.D. x 300 mm),
               (Right); Company A column
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : 1.0 mL/min
Detector     : UV (220 nm)
Column temp. : Room temp.

Contact us