Basic polypeptide Poly-Lysine was analyzed using PROTEIN KW-803 (a column for GFC separation).




Sample : 100 μL
Poly-L-lysine hydrobromide (Sigma-Aldrich) 0.1 %


Column       : Shodex PROTEIN KW-803 (8.0 mm I.D. x 300 mm)
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : 1.0 mL/min
Detector     : UV (220 nm) 
Column temp. : 30 °C

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