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Human IgM (2)

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IgM from human serum was analyzed using aqueous solvent SEC (GFC) columns, OHpak SB-805 HQ and SB-806 HQ.


Sample : 50μL
IgM, Immunoglobulin M from Human Serum
(Wako Pure Chemical Industries, Ltd.) 0.1%


Columns      : Shodex OHpak SB-806 HQ + SB-805 HQ (8.0mmI.D. x 300mm each)
Eluent       : 100mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate    : 1.0mL/min
Detector     : UV(254nm) 
Column temp. : 30°C

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