IgM from human serum was analyzed using aqueous solvent SEC (GFC) columns, OHpak SB-805 HQ and SB-806 HQ.

Sample : 50 μL
IgM, Immunoglobulin M from Human Serum
(Wako Pure Chemical Industries, Ltd.) 0.1 %

Columns      : Shodex OHpak SB-806 HQ + SB-805 HQ (8.0 mm I.D. x 300 mm each)
Eluent       : 100 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : 1.0 mL/min
Detector     : UV (254 nm) 
Column temp. : 30 °C

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