Calibration Curves for GF-HQ Series (Aqueous Eluent: Proteins)

Calibration curves for Asahipak GF-510 HQ and GF-7M HQ (Aqueous/Organic (multi-solvent use) SEC columns) are shown.


Sample:
1. IgM
2. IgA
3. IgG
4. Transferrin
5. Conalbumin
6. BSA
7. Ovalbumin
8. Myoglobin
9. Ribonuclease A
10. Cytochrome c
11. L-Phenylalanin
12. Creatinine

Column       : Shodex Asahipak GF-510 HQ (7.5 mm I.D. x 300 mm)       
Eluent       : 0.1 M Sodium phosphate buffer (pH7.0) + 0.1 M NaCl
Flow rate    : 0.5 mL/min
Detector     : UV (280 nm)
Column temp. : 30 °C

Column       : Shodex Asahipak GF-7M HQ (7.5 mm I.D. x 300 mm) 
Eluent       : 0.2 M Sodium phosphate buffer (pH7.0)
Flow rate    : 0.5 mL/min
Detector     : UV (280 nm)
Column temp. : 30 °C