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Comparison of Separation of BSA Aggregates


BSA and its aggregates were separated with PROTEIN LW-803 which is an aqueous SEC (GFC) column, and a conventional column (KW-803). With KW-803, the tetramer can be detected, however, the pentamer can be detected with LW-803. It is confirmed that LW-803 provided a better separation of the monomer and dimer than KW-803.

Sample : 5μL
BSA 24mg/mL (SIGMA A1900 BSA monomer)
1. Pentamer
2. Tetramer
3. Trimer
4. Dimer
5. BSA monomer (MW 66,000)

Columns      : Shodex PROTEIN LW-803 (8.0mmI.D. x 300mm)
               Shodex PROTEIN KW-803 (8.0mmI.D. x 300mm)
Eluent       : 50mM Sodium phosphate buffer (pH7.0) + 0.3M NaCl
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : 25°C

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