Ten kinds of protein standards were analyzed using KW403-4F
(a semi-micro column for aqueous SEC (GFC)).
Sample : 5μL
1. Thyroglobulin
(MW: 669,000)
2. γ-Globulin
(MW: 158,000)
3. BSA
(MW: 67,000)
4. Ovalbumin
(MW: 43,000)
5. Peroxidase
(MW: 40,200)
6. β-Lactoglobulin
(MW: 35,000)
7. Myoglobin
(MW: 17,000)
8. Ribonuclease A
(MW: 13,700)
9. Aprotinin
(MW: 6,500)
10. Vitamin B12
(MW: 1,350)
Column : Shodex KW403-4F (4.6mmI.D. x 300mm) Eluent : 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl Flow rate : 0.33mL/min Detector : UV(220nm) (small cell volume) Column temp. : 30°C
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