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Recovery of Proteins (KW-803)

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Both PROTEIN KW-803 and Company A column (a column for aqueous SEC (GFC)) show high mass recoveries of ribonuclrease A and thyroglobulin. Such high recoveries are attributed to minimum adsorption properties. Furthermore, the calibration curve obtained by sample loading and peak areas shows good linearity in a wide range between 1μg to 100μg.

Sample Column 1μg 5μg 10μg 50μg 100μg
Ribonuclease A KW-803 108.6 96.3 98.2 98.8 99.0
Company A 110.5 100.6 97.7 98.3 98.9
Thyroglobulin KW-803 106.1 98.2 97.3 98.7 98.6
Company A 111.9 92.6 93.9 95.9 96.6


 

Columns      : Shodex PROTEIN KW-803 (8.0mmI.D. x 300mm), Company A column
Eluent       : 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate    : 1.0mL/min
Detector     : UV(220nm)
Column temp. : Room temp.

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