Recovery of Proteins (KW-803)

Both PROTEIN KW-803 and Company A column (a column for aqueous SEC (GFC)) show high mass recoveries of ribonuclrease A and thyroglobulin. Such high recoveries are attributed to minimum adsorption properties. Furthermore, the calibration curve obtained by sample loading and peak areas shows good linearity in a wide range between 1 μg to 100 μg.
 

Sample Column 1 μg 5 μg 10 μg 50 μg 100 μg
Ribonuclease A KW-803 108.6 96.3 98.2 98.8 99.0
Company A 110.5 100.6 97.7 98.3 98.9
Thyroglobulin KW-803 106.1 98.2 97.3 98.7 98.6
Company A 111.9 92.6 93.9 95.9 96.6 

Columns      : Shodex PROTEIN KW-803 (8.0 mm I.D. x 300 mm), Company A column
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : 1.0 mL/min
Detector     : UV (220 nm)
Column temp. : Room temp.

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