Citric Acid Analysis in Functional Beverage (KC-811)

Citric acid is a functional food component which is effective in reducing fatigue in daily life and after exercise when regularly taken. Citric acid in a commercial functional beverage was analyzed using an organic-acids-analysis column, RSpak KC-811. A good linear calibration was obtained for the concentration range of 1 to 1,000 mg/L. Generally, a highly selective post-column method is required for the analysis of organic acids in foods, which contain many components such as food. However, for the analysis of functional beverages, a UV detector was effective for detecting the target, citric acid, without the influence of other components. The functional beverage can be analyzed after a simple dilution.

Sample preparation

  1. (1)Dilute a commercial citric acid-containing functional beverage 50-fold with water.
  2. (2)Filer using a 0.45-µm membrane filter and use it as an injection sample.

Sample: 5 µL
Standard solution

  1. 1.Citric acid  1000 mg/L

Commercial functional drink

  1. 1.Citric acid
  2. 2.Acetic acid
Shodex RSpak KC-811 (8.0 mm I.D. x 300 mm) x 2
3 mM HClO4 aq.
Flow rate
1.0 mL/min
UV (210 nm)
Column temp.
50 ℃

Sample Name Index

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

Product Name Index


Applications (Related Information)