Citric acid is a functional food component which is effective in reducing fatigue in daily life and after exercise when regularly taken. Citric acid in a commercial functional beverage was analyzed using an organic-acids-analysis column, RSpak KC-811. A good linear calibration was obtained for the concentration range of 1 to 1,000 mg/L. Generally, a highly selective post-column method is required for the analysis of organic acids in foods, which contain many components such as food. However, for the analysis of functional beverages, a UV detector was effective for detecting the target, citric acid, without the influence of other components. The functional beverage can be analyzed after a simple dilution.
- (1)Dilute a commercial citric acid-containing functional beverage 50-fold with water.
- (2)Filer using a 0.45-µm membrane filter and use it as an injection sample.
Sample: 5 µL
- 1.Citric acid 1000 mg/L
Commercial functional drink
- 1.Citric acid
- 2.Acetic acid
- ：Shodex RSpak KC-811 (8.0 mm I.D. x 300 mm) x 2
- ：3 mM HClO4 aq.
- Flow rate
- ：1.0 mL/min
- ：UV (210 nm)
- Column temp.
- ：50 ℃
Sample Name Index
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