Separation of Succinic Acid and Fumaric Acid (KC-811)

Succinic acid and fumaric acid were analyzed using RSpak KC-811. The elution time of succinic acid and fumaric acid largely changes according to eluent pH and column temperature. And, sometimes their peaks interfere the peaks of other substances. Comparing (1), (2) and (3), it was confirmed that the elution order of succinic acid and fumaric acid changes according to the conditions.
Please refer to "Effects of Column Temperature on Elution Volume (1) (KC-811)"


  1. 1.Succinic acid
  2. 2.Fumaric acid
  3. 3.Lactic acid
  4. 4.Formic acid
Shodex RSpak KC-G 8B (8.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 mm) x 2
(1); 2 mM HClO4 aq.
(2); 2 mM HClO4 aq./CH3CN=90/10
(3); 4 mM HClO4 aq./CH3CN=90/10
Flow rate
1.0 mL/min
VIS (430 nm) *Post column method
Column temp.
60 ℃

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