Elution Volume of Organic Acids (DE-413)

Elution volume of organic acids using RSpak DE-413 is shown.

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Substances Elution Volume (mL) Substances Elution Volume (mL)
Oxalic acid 1.75 Maleic acid   3.36
Glyoxylic acid 1.91 Acetic acid   3.37
DL-Tartaric acid 2.17 α-Ketoglutaric acid   3.55
Glycolic acid 2.22 Succinic acid   4.70
β-Hydroxypropionic acid 2.61 Levulinic acid   6.64
Formic acid 2.68 Propionic acid   7.12
Malic acid 2.72 Itaconic acid   8.94
Pyruvic acid 2.74 trans-Aconitic acid 10.80
Lactic acid 2.98 Fumaric acid 11.09
L-Pyroglutamic acid 3.07 Adipic acid 16.38
Citric acid 3.12 Mesaconic acid 28.48
Malonic acid 3.18
Column
Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent
10 mM H3PO4 aq.
Flow rate
1.0 mL/min
Detector
RI
Column temp.
50 ℃

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