Effects of Acetonitrile in Eluent (2) (KC-811)

When analyzing organic acids using RSpak KC-811, the elution time of organic acids with strong hydrophobicity can be shortened by adding 10 % acetonitrile to the eluent.


  1. 1.Succinic acid
  2. 2.Formic acid
  3. 3.Lactic acid
  4. 4.Acetic acid
  5. 5.Propionic acid
  6. 6.Isobutyric acid
  7. 7.n-Butyric acid
  8. 8.Isovaleric acid
  9. 9.n-Valeric acid
Shodex RSpak KC-G 8B (8.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 mm) x 2
2 mM HClO4 aq. or 2 mM HClO4 aq./CH3CN=90/10
Flow rate
(Eluent); 1.0 mL/min, (Reagent); 1.1 mL/min
VIS (430 nm) *Post column method
Column temp.
47 ℃

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