Elution Volume of Aromatic Organic Acids (NN-814)

RSpak NN-814, which has a higher polarity gel than that of RSpak KC-811 is suitable for the analysis of aromatic organic acids. The ion exclusion and reversed phase modes contribute to the separation of aromatic organic acids.

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Substances Elution Volume (mL) Substances Elution Volume (mL)
Shikimic acid 10.1 Benzoic acid 51.0
DL-Mandelic acid 19.2 Caffeic acid 66.9
Phthalic acid 19.8 Phenylpropionic acid 68.4
Trimellitic acid 23.0 Trimesic acid 69.0
m-Hydroxybenzonic acid 46.5 Salicylic acid 71.4
Vanillic acid 47.1 m-Toluic acid 86.4
p-Hydroxybenzoic acid 47.6 Ferulic acid 87.0
o-Hydroxybenzoic acid 48.8 p-Coumaric acid 90.3
3-Phenylpropionic acid 49.7
Column
Shodex RSpak NN-814 (8.0 mm I.D. x 250 mm)
Eluent
0.1 % H3PO4 aq.
Column temp.
60 ℃

RSpak NN-814, which has a higher polarity gel than that of RSpak KC-811 is suitable for the analysis of aromatic organic acids. The ion exclusion and reversed phase modes contribute to the separation of aromatic organic acids.

 
Substance Elution Volume (mL) Substance Elution Volume (mL)
Shikimic acid 10.1 Benzoic acid 51.0
DL-Mandelic acid 19.2 Caffeic acid 66.9
Phthalic acid 19.8 Phenylpropionic acid 68.4
Trimellitic acid 23.0 Trimesic acid 69.0
m-Hydroxybenzonic acid 46.5 Salicylic aci 71.4
Vanillic acid 47.1 m-Toluic acid 86.4
p-Hydroxybenzoic acid 47.6 Ferulic acid 87.0
o-Hydroxybenzoic acid 48.8 p-Coumaric acid 90.3
3-Phenylpropionic acid 49.7
Column       : Shodex RSpak NN-814 (8.0 mm I.D. x 250 mm)
Eluent       : 0.1 % H3PO4 aq.
Column temp. : 60 °C

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